Molecular markers associated with body weight of mirror carp and selection and raising of progenies

It is the one of main directions for aquaculture breeding using molecular markers that stocks were selected by the results of quantitative traits loci of economic traits. The main goal of our long term project is to get a good variety with most economic traits in high quality by marker-assisted selection. We have identified 6 microsatellite loci linked with body weight at high explained variation, and also found three of these 6 loci are homozygous in experimental population in our previous works. In this paper, using other three loci (HLJ302, HLJ338, HLJ343) which associated with body weight of mirror carp in breeding population, a group of mature mirror carp were selected and their progenies were also gotten. When the progenies grow up, the genotypes of those progenies were analyzed with these three loci. This paper compared the genotypes between 10 biggest fish and 10 smallest fish, the results showed that there are significant difference on dominant genotype linked body weight between fast-growth population and slow-growth population. The results showed that 10 biggest fish have the mean positive genotypes 1.7 for body weight. Meanwhile, which proved these markers are effect for breeding again. A big population with these positive dominant genotypes has been selected (also selected by good phenotypes such as good color, good body figure etc), and a big group of progenies of second generation has been produced. A new strain of fast-growing and good-quality mirror carp has been raised.