RAPD analysis on genetic variance of F1 of Hyriopsis cumingii from three populations

With the random amplified polymorphic DNA (RAPD) analysis method, forty ten-base arbitrary primers were used to study genetic variance of 9 F1 stocks produced from hybriding three geographical populations of Hyriopsis cumingii from Dongting Lake(DD), Poyang Lake(PP) and Taihu Lake(TT). The amplified results of thirteen primers were stably and clearly endowed with abundant stock polymorphy, and each primer could gain 2-17 fragments (200-3000 bp) in the 40 primers. Proportion of polymorphy was from 55% to 100%. AMOVA of 9 stocks indicated that 7.15% genetic variance was from intra-stock and 92.85% from inter-stock. Fixation index (Fst), the three lower were DP and PD(0.013)，TP and TT(0.020) and TP and TD(0.026)，the 4 higher were as follows: PT and DT(0.165)>TT and PT(0.153) >〖JP〗TD and PT (0.150)> DD and PT(0.137). Inter stock gene diversity (π) was as follows:TP(0.335)>TD(0.317)>TT(0.308)>PD(0.300)>DP(0.298)>PP(0.297)>DT (0.291)>PT(0.291)>DD(0.269).