Identification and properties of major allergen from Chinese shrimp, Penaeus chinensis

In order to obtain relevant data of Chinese shrimp allergen, and lay theoretical base for the allergen control, here the antigenic components and properties of Chinese shrimp were investigated. Its protein profiles were analyzed using SDS-PAGE. Then western-blotting was used to determine th e major allergen of it. A new shrimp allergen was obtained with high erformance liquid chromatography (HPLC), and its characterization was preliminarily studied by β elimination, UV a scan, peptide mass fingerprint (PMF) and IR spectrum. The result revealed that the major allergen of Chinese shrimp was molecules-36 ku protein. It was a kind of glycoprotein and the total carbohydrate content was 4.4%. The bond between the polysaccharide and the protein was O-type glycopeptides linkage. The major allergen of Chinese shrimp showed high sequence identity with that of other shellfish.