A cDNA library of Vibrio alginolyticus constructed by restriction display-PCR

A cDNA library of Vibrio alginolyticus mRNA with poly(A) tracts was constructed and identified by restriction display- polymerase chain reaction (RD-PCR). After being selected and screened, bioinformatics of 53 clones were analyzed. The results showed that some genes such as translocation protein in type III secretion, type III secretory pathway component EscU, putative chemotaxis transducer and secreted trypsin-like serine protease were identified, which indicated a high quality cDNA. The applied value of RD-PCR in construction of cDNA library of Vibrio alginolyticus mRNA with poly(A) tracts was also analyzed in this research. Key words:cDNA library; restriction display-PCR(RD-PCR); Vibrio alginolyticus