The effect of experimental cadmium poisoning on hepatopancreas, kidney, skeleton in Ctenopharyngodon idella

Experimentalcadmium (Cd) poisoningwasconductedon 200 grasscarpwithweightof 10.8g ?1. 2g. The content of Cd in the water of each experimental group was 80μg?L -1 for 1/ 10 LC50 group , 16μg?L -1for 1/ 50 LC50 group ,8μg?L -1 for 1/ 100 LC50 group , the control group < 1μg?L -1. This experiment lasted 120 days. The results indicated (1) the 24h LC50 in grass carp was 800μg Cd?kg -1weight. (2)themortalityratesin 1/10 LC50 group, 1/50 LC50 group, 1/100 LC50 group and controlgroup were: 64 %, 24 %, 20 %, 4%. (3) the contents (wet) of Cd in hepatopancreas , kidney , vertebra in experimental groups were very significantly higher than those of control group ( P <0.01). (4) the contents of glucose (GLU ), cholesterol (CHOL )glutamic2 oxalacetic2transaminase (GOT ) , alkalinephosphatease (ALP ) in blood plasma in experimental groups were significantly higher than those of control group ( P < 0 . 05 ) , but total protein ( TP ) is lower significantly ( P <0.05). (5) the contents (wet ) of Ca in vertebra and Ca, P, Mg in serum in experimental groups were significantly lower than those of control group ( P < 0. 05) . (6) The pathology showed that hepatocytes , nenal tubule and globule epithelial cells were granular degeneration , hydropic degeneration and fatty degeneration , dissolved necrosis , cyrtosis , silks of gill sinked , osteodysplasty. Ultrastructural changes showed that mitochondria in hepatocytes and kidney epithelial cells were swollen with disintegration and lysis of cristaes , endoplasmic reticulum was hyperplastic and fragemented. The thickness of basal membrane of the kidney glomerulus was found.