Expression of the envelope protein gene vp28 of the white spot syndrome virus in Pichia pastoris

A pair of primers was designed based on the vp 28 gene and PCR was performed to amplify the gene f rom WSSV DNA. Insert ing the DNA fragment to the yeas-t E . coli shuf fle vector pPICZ and the recombinant plasmid ( pPICZVP28) that contains the target DNA fragment was obtained in the E. coli strain. The pPICZVP28 was introduced into Pichia pastoris strain X33 by electroporation. The transformant strain X33-1 was grown in BMGY media in fled f lask at 28 e . Induced by methanol for 72h, the samples of cell pellets and supernatant were collected by centrifugation and analyzed by SDS-PAGE and Western-blot, which confirmed that the strain X33-1 can express the WSSV. s envelope protein vp 28.