Detection of baculovirus infection in wild..caught Penaeus chinensis by PCR and dot blot hybridization

Wild..caught Penaeus chinensis was detected for baculovirus by Polymerase Chain Reaction( PCR) and dot blot hybridization using DIG..labeled Penaeus chinensis Baculovirus ( PCBV) fragment of nuclein lengthened 800, 1 100, 1 500 and 2 500bp. Positive reaction was found in gills, hepatopancreas, midgut and ovaries , but muscular tissue was negative. Our results demonstrated that the ovaries of shrimps were infected by the baculovirus that implied the virus may be transmitted vertically from parent to young generation.