Wu Tingting, Yang Hong, Dong Zaijie, Xia Dequan, Shi Yingxian, Ji Xiaodong, Shen Yu, Sun Weiyong. THE INTEGRATION AND EXPRESSION OF HUMAN GROWTH GENE IN BLUNT SNOUT BREAM AND COMMON CARP[J]. Journal of fisheries of china, 1994, 18(4): 284-289.
Citation: Wu Tingting, Yang Hong, Dong Zaijie, Xia Dequan, Shi Yingxian, Ji Xiaodong, Shen Yu, Sun Weiyong. THE INTEGRATION AND EXPRESSION OF HUMAN GROWTH GENE IN BLUNT SNOUT BREAM AND COMMON CARP[J]. Journal of fisheries of china, 1994, 18(4): 284-289.

THE INTEGRATION AND EXPRESSION OF HUMAN GROWTH GENE IN BLUNT SNOUT BREAM AND COMMON CARP

  • Linealized DNA fragments recombined out of the mouse gene promotor MT-1 and human growth hormone gene were microinjected into the fertilized eggs of blunt snout bream (Megalobrama amblycephala) and common carp (Cyprinus carpio) respectively. By the inspecting methods of the dot blot,the Southern blot,the radioactive immunities and the enzymelinked immunosorbent assay,the integration,transcription,expression and growth promoting effect of the foreign genes in the receptive fish were confirmed. The sexually-reproduced F1 offsprings from transgenic male carps and female carps carry the foreign genes,thus showing that the foreign genes can be transmitted into F1 offsprings and have growth promoting effect.
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