ESTABLISHMENT OF CELL STRAIN AHZC 88 RESISTANT TO PATHOGENIC VIRUSES OF GRASS CARP HEMORRHAGE BY ULTRAVIOLET (UV) INDUCEMENT

The cell strain AHZC 88 of anti-hemorrhage of grass carp has been obtained by repeatedly UV inducement on strain ZC 7901 from the snout tissue cell sensitive to hemorrhage of grass carp. Identification of its biological characteristic showed that its chromosome number was 2n=48,the range of its suitable temperature was between 4-38℃, the optimal growth was 27℃, and mitotic index reached to maximum in 36 hours. The infective test with the pathogenic virus of hemorrhage proved that the cell strain AHZC 88 was not infected with the hemorrhage virus. The observation under electron microscope showed that there was no virosome in the cell of strain. The analysis of LDH isoenzyme displayed five zones which were different from the sensitive cell strain.