A RAPID PREPARATION OF FATTY ACID METHYL ESTERS FOR GAS CHROMATOGRAPHIC ANALYSIS

A rapid preparation of fatty acid methyl esters by using direct reactionin the ground sample without lipid extraction was developed. Fish flesh, fish viscera,fish liver. fish eggs and dried shrimps were used as samples in comparative experi-ments. The results of this new direct method were extremely close to those of or-dinary method in which lipid was extracted according to Bligh-Dyer's method andfatty acids was esterified according to Metcalfe's method (tt_(0.05). The advantagesof this direct method are rapid, reagent saving, simple and convenient. It can solvethe difficulty in extracting lipid from samples of small amount or of less in lipid inthe case of ordinary method.