Effects of different delayed first feeding time on fatty acid and amino acids of Myxocyprinus asiaticus larvae

To investigate the effect of different delayed first feeding time on the amino acids and fatty acids of Myxocyprinus asiaticus larvae under the condition of water temperature(19±0.5)℃,we divided the larvae into 7 different initial delayed feeding time groups,1,3,5,7,9 and 11 delayed days denoted as group D0,D1,D3,D5,D7,D9 and D11 respectively,and subsequently fed at corresponding time,then detected the composition and content of the amino acids and fatty acid at different intervals,with total experimental period lasting 29 days.Results showed that,(1)The larvae body lipid content showed a significant downward trend when prolonging the starvation time.However,at the end of the experiment(29 days old),all of the other groups were not significantly different from the control group on lipid content,indicating a complete compensation effect.(2)During starvation,the monounsaturated fatty acids(MUFA)were utilized as energy substrates by M.asiaticus larvae,with the order of n-6>n-9>n-3 and C22∶6n-3(DHA)being preserved prior to C20∶5n-3(EPA).In addition,the contents(percentage)of DHA and ARA were significant different from the body weight and moisture content.(3)With increasing time of starvation,the alanine,isoleucine,leucine and histidine decreased significantly,while the aspartic acid and glutamic acid increased significantly,however,the glycine,lysine and arginine first increased and then decreased in M.asiaticus larvae.To sum up,first,M.asiaticus larvae have strong adaptability for starvation,and mainly consume lipids in the early starvation period,and then amino acids begin to be consumed when the lipids come near to the threshold.Then,it suggested a slower restoration of the amino acid than the fatty acids for M.asiaticus larvae after resuming feeding.