Development and application of three multiplex PCR panels of microsatellites in large yellow croaker(Larimichthys crocea)

In this study, three multiplex PCR panels were established using microsatellite markers developed from our laboratory by optimizing annealing temperature, concentration of primers and times of cycle.A breeding group "JD-01" was analyzed using the three multiplex PCR panels.The results showed:the average number of alleles and effective number of alleles were 12.111 and 7.408 respectively.The average of polymorphism information content(PIC) were 0.846.The average of observed heterozygosity(H_o) and expected heterozygosity(H_e) was 0.825 and 0.868, respectively.The Shannon's diversity index was 2.165.By using Cervus 3.0 software, we verified parentage assignment that the pedigree relationship was known in 9 families of large yellow croaker.As a result, the accuracy rate of parentage assignment was 100% by using three multiple PCR panels.The development of microsatellite multiple PCR system provides a high efficiency technical method for genetic diversity analysis, parentage assignment and marker-assisted family management in large yellow croaker.