Cloning and expression analysis of two cDNAs encoding lipoprotein lipase of Micropterus salmoides

To promote the theoretical basic research on developing artificial feed for carnivorous fishes,and the analysis of fat metabolizing mechanism in fish,the cDNA of LPLtype1 and LPLtype2 genes from largemouth bass(Micropterus salmoides)are cloned.The sequence analysis indicates that LPLtype1 cDNA is 2 156 bp in length,encoding 516 amino acids;and LPLtype2 cDNA is 1 710 bp in length,encoding 346 amino acids.The amino acid sequence of LPLtype1 is 43.5% identified to that of LPLtype2.Phylogenetic analysis indicates that LPLtype1 of M.salmoides and LPL of Siniperca chuatsi gather to one branch.LPLtype2 of M.salmoides and LPLtype2 of Oncorhynchus clarkii gather to the other branch.The predictive analysis of the protein encoded by LPLtype1 and LPLtype2 genes in M.salmoides shows that the main functional regions are conservative compared with the bony fishes and other vertebrates,such as active site residue,N-glycosylation site,conserved hydrophobic interaction site,heparin binding region.The tissue expression levels of LPL mRNA are detected by real time PCR,and the results show that expression levels of LPLtype1 and LPLtype2 are the highese in liver,which suggests that fish liver plays an essential role in storing lipids induced by nutrition.