Analysis of genomic structure and the promoter characterization of FcβGBP-HDL from Fenneropenaeus chinensis

FcβGBP-HDL was a pattern recognition receptor(PRR)which was up regulated post WSSV(white spot syndrome virus)challenge.To investigate the mechanism of transcription regulation,primers were designed based on the FcβGBP-HDL cDNA obtained,the gene and 5′-flanking sequences were isolated from the Chinese Shrimp Fenneropenaeus chinensis by genome walking,and the activity of promoter was analyzed by voter construction.The results showed that this gene was composed of only one exon spanning 6 713 bp.The 5′-flanking sequence(1 507 bp)of FcβGBP-HDL gene contained several typical transcription regulation elements,such as one promoter core sequence,two SRF,two TBP,one CTF and one CRE,and several transcription regulation elements of immune-gene in other arthropods,such as two GATA 1,three AP 1 and seven Sp1.The upstream sequence of FcβGBP HDL gene had highest level similarity with FcCTL(similarity 41.4%).The transcription levels of luciferase reporter gene were up regulated in all construction voters,highest in pGL3-FcβGBP2 with the deletion of two AP-1,and lowest in pGL3-FcβGBP4 with the deletion of promoter core sequence.The results indicated that promoter of FcβGBP-HDL might be regulated in immune reaction.