Response surface methodology for optimization of growth condition in juvenile Ruditapes philippinarum

In this paper,the optimum temperature,salinity and pH for juvenile growth of Ruditapes philippinarum were determined through the response surface methodology(RSM).The multivariate non-linear model was applied to study the interactions and optimization of the growth parameters for R.philippinarum which were explored by experiments.The results showed that:(1)there were significantly positive correlations between growth of shell-length and temperature and salinity within the range of experiment design.The relationship between growth of shell length and temperature and salinity and the growth of shell-length verged to positive correlation,and the calm grew better between pH 8 and 9;(2)there were no significant interactions between temperature,salinity and pH for the growth of juveniles.The multivariate non-linear model is as follows:Y=4.08+0.10A+0.18B+0.11C-0.055A²-0.13B²-0.12C²(R²=0.977 4),the optimum conditions for germination are as follows:temperature 29.65 ℃ and salinity 23.35‰ and pH=8.46±0.09.The experimental results indicated that the proposed model is reasonably accurate and can be used for describing and predicting the growth features ofR.philippinarum in different environmental factors.