Prokaryotic expression, antibody preparation and tissue expression identification of tropomyosin in Marsupenaeus japonicas

Food allergy is?a?representative?human?allergic disease,?which is mainly?caused?by?the protein in food. Up to date, however, there is only a few reports on aquatic?food?allergy. Tropomyosin(TPM) is the major allergen of eating seafood like shimp and crab to cause hypersensitivity. In this investigation, a TPM gene was cloned from the muscle tissue of shrimp, Marsupenaeus japonicus. Then the TPM was expressed in Escherichia coli and purified. Subsequently the specific antibody was raised using the purified fusion protein (GST-TPM). As revealed by Western-blotting, the TPM was ubiquitously expressed in shrimp tissues and with the highest expression in the muscle and the lowest in gill and skin. These results have laid the foundation?for the specific diagnosis and immunotherapy in shrimp hypersensititivity disease as well as the study on food desensitization.