Effect of ascorbic acid on hepatocytes viability and antioxidant capability of crucian carp (Carassius auratus gibelio) in vitro

In the present study,the effect of ascorbic acid on primary cultured hepatocytes viability and antioxidant capability under trichlorfon stress were investigated in Carassius auratus gibelio. The hepatocytes were cultured with media containing 0,50,100,200,400 and 800 μmol/L concentration ascorbic acid.Cell viability,the changes in hepatocytes lactate dehydrogenase(LDH)activity and ascorbic acid concentration were assayed.In addition,cellular intracellular total antioxidant capacity(T-AOC),glutathione-S-ePoxide transferase(GST)and butyrylcholinesterase(B-ChE)activities and cytochrome P450(CYP450)concentration under trichlorfon stress were determined.The results showed that hepatocyte viability was significantly increased(P<0.05)when cell was cultured with 100 μmol/L concentrations ascorbic acid in vitro compared with control.Cellular ascorbic acid concentration was significantly increased with media contained 50,100,200,400 and 800 μmol/L concentration ascorbic acid.There were no significant changes in cellular LDH activity with media containing 50,100,200,400 μmol/L concentration ascorbic acid.But LDH activity was markedly(P<0.05)increased when ascorbic acid concentration was 800 μmol/L.Under trichlorfon tress,the cellular antioxidant capability was increased significantly(P<0.05)at 50,100 and 200 μmol/L concentration ascorbic acid treatments.In conclusion,ascorbic acid facilitated hepatocytes viability and function of anti-oxidative stress when cells were cultured with 50-200 μmol/L concentrations ascorbic acid.