Analysis of the karyotype and banding patterns of Verasper variegatus

The karyotype of Verasper variegatus was studied through the chromosome preparations obtained from head kidney by the method of injecting PHA and colchicines.Metaphase chromosomes were analyzed by means of C-banding,Ag-NORs and G-banding.Experiment results showed that the diploid chromosome number of V.variegatus was 46(2n=46t,NF=46).There was no visible evidence of sex chromosome and satellite chromosome.C-bands of V.variegatus were located mainly in the centromere regions of chromosomes except the No.19 and 22 chromosomes,and the 19th pair of chromosomes showed positive C-bands in the terminal region while the whole 22th pair of chromosomes bore positive C-band thoroughly.The C-banding heretochromatin accounts for 30% of the total chromosome surface in V.variegatus.Silver staining study showed that V.variegatus bore a pair of Ag-NORs which was located at the end of long arm of the 2nd chromosome.A total of 59 positive G-bands(38 dark,21 light)were recorded in 23 pairs of chromosomes.These results would provide basic data for the genomic research and biodiversity conservation of V.variegatus.