Construction of DNA fingerprinting database of germplasm materials of Porphyra haitanensis

20 simple sequence repeat(SSR)primer-pairs were used to analyze the genetic diversity of 44 germplasm materials of Porphyra haitanensis stored in the germplasm repository.A total of 81 alleles were detected,with the number of alleles ranging from 2 to 6,and the average was 4.05,and the polymorphism information content(PIC)was between 0.15 and 0.79,with the average of 0.57.Then the 16 polymorphic alleles amplified from 3 primer-pairs of SSR(Phes08,Phes03 and PC13)were used to construct the DNA fingerprinting dabatase of the 44 germplasm materials of P.haitanensis.In the constructed DNA fingerprinting database,each germplasm material has its unique fingerprinting pattern and can be easily distinguished from each other.At last the DNA fingerprints were transformed into digital fingerprints which can be recognized by computer based on appear ance and disappear ance of amplified band in each allele,and developed the corresponding software of digital fingerprints recognizer to predigest the germplasm identified process.In conclusion,the fingerprinting database will provide a basis for germplasm auto-identification and digital management of germplasm repository of P.haitanensis.