• ISSN 1000-0615
  • CN 31-1283/S
PENG Aihong, GAO Shuang, CHEN Jun, ZHUANG Meizhen, WANG Fan, CHEN Xiaomei, LIN Zhengzhong, HUANG Zhiyong. Growth exhibition and metallothioneins induction of Chlorella sp. exposed to different zinc salts[J]. Journal of fisheries of china, 2017, 41(7): 1126-1132. DOI: 10.11964/jfc.20160410349
Citation: PENG Aihong, GAO Shuang, CHEN Jun, ZHUANG Meizhen, WANG Fan, CHEN Xiaomei, LIN Zhengzhong, HUANG Zhiyong. Growth exhibition and metallothioneins induction of Chlorella sp. exposed to different zinc salts[J]. Journal of fisheries of china, 2017, 41(7): 1126-1132. DOI: 10.11964/jfc.20160410349

Growth exhibition and metallothioneins induction of Chlorella sp. exposed to different zinc salts

Funds: 

Natural Science Foundation of Fujian Province of China (2015J01615); Special Project of Xiamen Marine Economy Development of China (14GZP67NF31); Foundation for Innovative Research Team of Jimei University (2010A007)

More Information
  • Corresponding author:

    HUANG Zhiyong. E-mail: zhyhuang@jmu.edu.cn

  • Received Date: April 09, 2016
  • Revised Date: January 14, 2017
  • Available Online: November 26, 2017
  • Published Date: June 30, 2017
  • The exhibition of growth and the induction of metallothioneins of Chlorella sp. exposed to different kinds of zinc salts were investigated. The biomass, heat-stable proteins and Zn-MTs were measured when the Chlorella sp. were exposed to four kinds of Zn2+ (including zinc chloride, zinc acetate, zinc gluconate, zinc citrate) at the concentrations of 5, 10, 20, 50 and 100 μmol/L. Results showed that the growth of Chlorella sp. was not significantly inhibited when the zinc concentrations were less than 5 and 10 μmol/L for zinc citrate and zinc chloride, respectively. But the growth was significantly inhibited at the concentrations of 5 μmol/L for each of zinc acetate and zinc gluconate, and the algal biomass rapidly decreased with the increases of Zn2+ concentrations for the two zinc salts. The results also showed that the amounts of heat-stable proteins significantly increased in Chlorella sp. exposed to the four kinds of zinc salts compared with that of the control group (without adding any zinc salt), and the largest amount of heat-stable proteins was obtained when the algae were exposed to zinc gluconate at 50 μmol/L for 3 days, producing 34.5 mg of heat-stable proteins per gram of wet algae mud, which was 3.3 times higher than that of the control group. And the largest amount of Zn-MTs was induced under the inducing condition.
  • [1]
    张旗, 尚小玉, 王娜, 等. 小球藻营养活性研究进展[J]. 食品研究与开发, 2015, 36(13): 139-143.

    Zhang Q, Shang X Y, Wang N, et al. The research progress on nutrition activity of Chlorella[J]. Food Research and Development, 2015, 36(13): 139-143 (in Chinese).
    [2]
    Yang H, Huang Z Y, Li J, et al. MT-like proteins: potential bio-indicators of Chlorella vulgaris for zinc contamination in water environment[J]. Ecological Indicators, 2014, 45: 103-109.
    [3]
    李连平, 张小东, 黄志勇, 等. 小球藻锌结合类金属硫蛋白的结构表征及抗菌活性[J]. 水产学报, 2010, 34(2): 278-284.

    Li L P, Zhang X D, Huang Z Y, et al. Studies on the structural characterization and antimicrobial activities of Zn-MT-like proteins induced from Chlorella vulgaris[J]. Journal of Fisheries of China, 2010, 34(2): 278-284 (in Chinese).
    [4]
    李连平, 黄志勇, 王志聪, 等. 小球藻锌结合金属硫蛋白(Zn-MT-like)的抗氧化活性研究[J]. 中国食品学报, 2009, 9(4): 23-27.

    Li L P, Huang Z Y, Wang Z C, et al. Study on anti-oxidation activity of Zn-MT-like induced from C. Vulgaris[J]. Journal of Chinese Institute of Food Science and Technology, 2009, 9(4): 23-27 (in Chinese).
    [5]
    Huang Z Y, Li L P, Huang G L, et al. Growth-inhibitory and metal-binding proteins in Chlorella vulgaris exposed to cadmium or zinc[J]. Aquatic Toxicology, 2009, 91(1): 54-61.
    [6]
    李连平, 梁英, 黄志勇, 等. 小球藻锌结合类金属硫蛋白的提取和分离[J]. 食品与发酵工业, 2009, 35(6): 188-192.

    Li L P, Liang Y, Huang Z Y, et al. Extraction and separation of Zn-MT-like induced from C. Vulgaris under the stress of ZnCl2[J]. Food and Fermentation Industries, 2009, 35(6): 188-192 (in Chinese).
    [7]
    王文君, 吕娜, 尹锐, 等. 金属硫蛋白研究进展[J]. 江苏农业科学, 2016, 44(1): 13-16.

    Wang W J, Lü N, Yin R, et al. Research progress of metallothionein[J]. Jiangsu Agricultural Sciences, 2016, 44(1): 13-16 (in Chinese).
    [8]
    杨洪, 黄志勇. 锌胁迫对小球藻抗氧化酶和类金属硫蛋白的影响[J]. 生态学报, 2012, 32(22): 7117-7123.

    Yang H, Huang Z Y. Activities of antioxidant enzymes and Zn-MT-like proteins induced in Chlorella vulgaris exposed to Zn2+[J]. Acta Ecologica Sinica, 2012, 32(22): 7117-7123 (in Chinese).
    [9]
    刘学铭, 余若黔, 梁世中. 分批异养培养小球藻光密度值与干重的关系[J]. 微生物学通报, 1999, 26(5): 339-341.

    Liu X M, Yu R Q, Liang S Z. The relationship between optical density and dry weight of Chlorella vulgaris in batch heterotrophic culture[J]. Microbiology, 1999, 26(5): 339-341 (in Chinese).
    [10]
    沈萍萍, 王朝晖, 齐雨藻, 等. 光密度法测定微藻生物量[J]. 暨南大学学报(自然科学版), 2001, 22(3): 115-119.

    Shen P P, Wang Z H, Qi Y Z, et al. An optical density method for determination of microalgal biomass[J]. Journal of Jinan University (Natural Science Edition), 2001, 22(3): 115-119 (in Chinese).
    [11]
    邓丽莉, 潘晓倩, 生吉萍, 等. 考马斯亮蓝法测定苹果组织微量可溶性蛋白含量的条件优化[J]. 食品科学, 2012, 33(24): 185-189.

    Deng L L, Pan X Q, Sheng J P, et al. Optimization of experimental conditions for the determination of water soluble protein in apple pulp using Coomassie Brilliant Blue Method[J]. Food Science, 2012, 33(24): 185-189 (in Chinese).
    [12]
    Huang Z Y, Shen J C, Zhuang Z X, et al. Investigation of metal-binding metallothioneins in the tissues of rats after oral intake of cinnabar[J]. Analytical and Bio- analytical Chemistry, 2004, 379(3): 427-432.
    [13]
    李坤, 李琳, 侯和胜, 等. Cu2+、Cd2+、Zn2+对两种单胞藻的毒害作用[J]. 应用与环境生物学报, 2002, 8(4): 395-398.

    Li K, Li L, Hou H S, et al. Study on toxicity of heavy metal ions to two species of marine unicellular algae[J]. Chinese Journal of Applied & Environmental Biology, 2002, 8(4): 395-398 (in Chinese).
    [14]
    Bilgrami K S, Kumar S. Effects of copper, lead and zinc on phytoplankton growth[J]. Biologia Plantarum, 1997, 39(2): 315-317.
    [15]
    欧阳慧灵, 孔祥臻, 秦宁, 等. 5种重金属亚致死暴露浓度对普通小球藻生长抑制与光合毒性的比较[J]. 科学通报, 2012, 57(10): 785-793.

    Ouyang H L, Kong X Z, Qin N, et al. Effects of five heavy metals at sub-lethal concentrations on the growth and photosynthesis of Chlorella vulgaris[J]. Chinese Science Bulletin, 2012, 57(25): 3363-3370.
    [16]
    徐勤松, 施国新, 王学, 等. 镉、铜和锌胁迫下黑藻活性氧的产生及抗氧化酶活性的变化研究[J]. 水生生物学报, 2006, 30(1): 107-112.

    Xu Q S, Shi G X, Wang X, et al. Generation of active oxygen and change of antioxidant enzyme activity in Hydrilla verticillata under Cd, Cu and Zn stress[J]. Acta Hydrobiologica Sinica, 2006, 30(1): 107-112 (in Chinese).
    [17]
    常团结, 朱祯. 植物金属硫蛋白研究进展(一)—植物MT的分类、特征及其基因结构[J]. 生物技术通报, 2002(3): 5-10.

    Chang T J, Zhu Z. Study advances of plant metal- lothionein—classification, characteristics and gene structure[J]. Biotechnology Bulletin, 2002(3): 5-10 (in Chinese).
    [18]
    郭祥学, 陈正佳, 但春涛, 等. 聚球藻类金属硫蛋白的纯化及部分性质的研究[J]. 生物化学杂志, 1997, 13(6): 699-703.

    Guo X X, Chen Z J, Dan C T, et al. Purification and characterization of metallothionein-like protein in Synechococus sp. PCC 7942[J]. Chinese Biochemical Journal, 1997, 13(6): 699-703 (in Chinese).
    [19]
    Yoshida N, Ishii K, Okuno T, et al. Purification and characterization of cadmium-binding protein from unicelluar alga Chlorella sorokinian[J]. Current Microbiology, 2006, 52(6): 460-463.
    [20]
    Silvestre F, Duchêne C, Trausch G, et al. Tissue-specific cadmium accumulation and metallothionein-like protein levels during acclimation process in the Chinese crab Eriocheir sinensis[J]. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 2005, 140(1): 39-45.
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