Effects of temperature and salinity on embryonic and larval development of Haliotis ovina

In order to investigate the effect of temperature and salinity on Haliotis ovina embryonic and larval development in Wenchang Hainan during November to December, 2013, we used the artificial domestication of wild H. ovina as experimental material, and single or double factor combination method with the temperature and salinity to design the experiment. The results showed that: the hatching rate and the survival rate of H. ovina were significantly affected by temperature and salinity. When the temperature was 28℃, the embryonic development time was the shortest and the hatching rate was the highest. When the temperature was 26℃, the larval development time was the shortest and the survival rate was the highest. The range of suitable temperature for embryonic development was 24-28℃, and the optimal temperature was 26-28℃. The range of suitable temperature for larval development was 26-30℃, and the optimal temperature was 26-28℃. When the salinity was 24, the embryonic development time and the larval development time were the shortest but the hatching rate and the survival rate were the lowest. The hatching rate and the survival rate were the highest at 30 and 32, respectively. The range of suitable salinity for embryonic development and larval development was 28-32, and the optimal salinity was 30-32. There was no siginificant effect of temperature and salinity interation experiments on the hatching rate, but there was siginificant effect on the survival rate. Based on the response surface model, the hatching rate and the survival rate reached the maximum(95.32%, 81.20%) when the 2-factor combination was 24.61℃ and 32, with the desirability value being 0.848. The studies indicated that the H. ovina embryonic development and larval development had different sensitivity to different temperature and salinity, The high temperature and high salinity were better tolerated than the low temperature and low salinity.