LU Ronghua, YANG Feng, SUN Junjun, XIE Dizhi, QIN Chaobin, YANG Liping, ZHENG Wenjia, NIE Guoxing. Molecular cloning of PGC-1β gene and effects of high carbohydrate and high fat feed on its expression in liver of grass carp Ctenopharyngodon idella[J]. Journal of fisheries of china, 2015, 39(9): 1283-1290. DOI: 10.11964/jfc.20150109689
Citation: LU Ronghua, YANG Feng, SUN Junjun, XIE Dizhi, QIN Chaobin, YANG Liping, ZHENG Wenjia, NIE Guoxing. Molecular cloning of PGC-1β gene and effects of high carbohydrate and high fat feed on its expression in liver of grass carp Ctenopharyngodon idella[J]. Journal of fisheries of china, 2015, 39(9): 1283-1290. DOI: 10.11964/jfc.20150109689

Molecular cloning of PGC-1β gene and effects of high carbohydrate and high fat feed on its expression in liver of grass carp Ctenopharyngodon idella

  • To investigate the mechanism of peroxisome proliferator-activated receptor-γ coactivator-1 beta(PGC-1β)in the energy metabolism of grass carp Ctenopharyngodon idella,the partial cDNA of PGC-1β was cloned,and tissue expression and nutritional regulation were investigated.The partial cDNA was 885 bp (GenBank number:KM580493.1),encoding a polypeptide of 293 amino acids,compared to zebra fish,it showed 81% homology.Quantitative real-time PCR showed that highest PGC-1β mRNA expression was detected in brain, followed by intestine and liver.PGC-1β gene expression levels in liver were significantly increased during 7 days of fasting and decreased to normal level after refeeding.The expression of PGC-1β was significantly affected by dietary lipid and carbohydrate, significantly higher in liver of fish fed the diet with high carbohydrate and high lipid.The results indicate that PGC-1β is regulated by the energy expenditure status,which implies it plays an important role in energy metabolism in grass carp.
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