Cloning,spatiotemporal expression and SNPs identification of GRB2 gene in hard clam Meretrix meretrix

To explore the molecular structure and biological function of GRB2 in Meretrix meretrix,GRB2 cDNA was cloned by SMART RACE techniques,then the bioinformatics and expression profiles in different tissues and developmental stages were analyzed and SNPs were screened out in exon.The results indicated that the full length cDNA of GRB2 gene was 1 791 bp,containing a complete 669 bp ORF encoding 223 amino acids.There were three functional domains of GRB2protein(SH3-SH2-SH3).Comparisons of animal acid sequence,the GRB2 of M.meretrix has highly homologous with Tegillarca granosa and shares 65.6% similarity.It shares more than 60% similarity with vertebrates and proves that GRB2 was highly conservative.The result of qRT-PCR showed thatGRB2 expressed in all six tissues and ten developmental stages,but the expression of tissues did not have significantly difference.The relative expression in different stages revealed that the expression of GRB2 gradually increased with the process of the development,and showed the highest in umbo larvae stage.16 SNPs in the exon of GRB2 were identified.