| Citation: |
ZHOU Liqing, GE Guangyu, JING Hao, WU Zhou, SUN Xiujun, LI Jiale, WU Biao, LIU Zhihong, YANG Jinlong. Effects of dissolved oxygen changes on the gill tissue structure of Ruditapes philippinarum[J]. Journal of fisheries of china, 2024, 48(10): 109105. DOI: 10.11964/jfc.20231114218
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The Ruditapes philippinarum mainly inhabits estuaries, inner bays and coastal mudflat. Affected by tides, rainfall and seasons, the dissolved oxygen in their living environment is prone to change, which will affect their growth, survival and metabolism.The gills of bivalves are both respiratory organs and feeding organs used for filtering food. In order to investigate the effect of dissolved oxygen changes on the gill tissue structure of R. philippinarum and provide parameters for their aquaculture management, we designed three modes of dissolved oxygen changes, namely, continuous maintenance of regular dissolved oxygen C treatment, regular dissolved oxygen-rapid hypoxia for 24 h-rapid reoxygenation for 4 h AHR treatment, and regular dissolved oxygen-slow hypoxia for 48 h-slow reoxygenation for 8 h CHR treatment. Then tissue sections and immunohistochemstry methods were used to analyze the effect of dissolved oxygen changes on the gill tissue structure. The results of tissue sectioning showed that dissolved oxygen changes would affect the morphology and structure of gill tissue in R. philippinarum. Hypoxia caused gill filaments to widen, surface epithelial cells to damage, cilia to fall off, the sponge like blood cavity tissue to become loose, gill lumen gaps to become larger, and there were cell fragments inside. Hypoxia reoxygenation significantly changed the morphology of the gill flap, and the damage to the tissue cells of the outer gill flap was more severe than that of the inner gill flap. Slow hypoxia for 48 h and slow reoxygenation for 8 h caused more severe damage to the gill tissue structure than quick hypoxia for 24 h and quick reoxygenation for 4 h. The immunohistochemical results showed that both rapid hypoxia for 24 h and slow hypoxia for 48 h increase the ROS level in the gill tissue, while reoxygenation only reduced the ROS level a little in the gill tissue. There was no significant repair effect on the damage caused by hypoxia in a short period of time. The gill tissue cells damaged by slow hypoxia for 48 h and reoxygenation for 8 h were numerous and diffuse, with unclear nuclear cytoplasmic boundaries, the degree of damage to gill tissue cells was higher than that of rapid hypoxia for 24 h and reoxygenation for 4 h. In summary, this experiment found that both slow hypoxia for 48 h and rapid hypoxia for 24 h could cause damage to the cellular structure of the gill tissue. The damage to reoxygenated tissue cells could not be repaired in a short period of time. Slow hypoxia for 48 h caused more severe damage to gill tissue structure than fast hypoxia for 24 h. The tissue cell damage continues to worsen even after 8 hours of slow reoxygenation. The results of this study suggested that the management of R. philippinarum farming need to consider the stable control of dissolved oxygen, and efforts should be made to avoid changes in dissolved oxygen, especially the damage to the gill tissue of clams caused by long-term chronic hypoxia stress. This study provides parameters for dissolved oxygen control in the process of R. philippinarum aquaculture.
| [1] |
Li Q, Sun S, Zhang F, et al. Effects of hypoxia on survival, behavior, metabolism and cellular damage of Manila clam (Ruditapes philippinarum)[J]. PLoS ONE, 2019, 14 (4): e0215158.
|
| [2] |
Barnett A F, Gledhill J H, Griffitt R J, et al. Combined and independent effects of hypoxia and tributyltin on mRNA expression and physiology of the eastern oyster (Crassostrea virginica)[J]. Scientific Reports, 2020, 10: 10605. doi: 10.1038/s41598-020-67650-x
|
| [3] |
孙虎山, 王晓安. 紫彩血蛤鳃的组织化学和扫描电镜研究[J]. 动物学杂志, 1999, 34 (4): 9-12. doi: 10.3969/j.issn.0250-3263.1999.04.003
Sun H S, Wang X A. Histochemical and electron scanning microscopic studies on the gills of Nuttallia olivacea[J]. Chinese Journal of Zoology, 1999, 34 (4): 9-12(in Chinese). doi: 10.3969/j.issn.0250-3263.1999.04.003
|
| [4] |
宋晓楠, 马峻峰, 秦艳杰, 等. 盐度骤降对菲律宾蛤仔抗氧化酶活力及组织结构的影响[J]. 农学学报, 2013, 3 (1): 50-56. doi: 10.3969/j.issn.1007-7774.2013.01.011
Song X N, Ma J F, Qin Y J, et al. Effects of abrupt decline in salinity on the antioxidant enzyme activities and histological structure in Ruditapes philippenarum[J]. Journal of Agriculture, 2013, 3 (1): 50-56 (in Chinese). doi: 10.3969/j.issn.1007-7774.2013.01.011
|
| [5] |
Zhang Y, Wu H F, Wei L, et al. Effects of hypoxia in the gills of the Manila clam Ruditapes philippinarum using NMR-based metabolomics[J]. Marine Pollution Bulletin, 2017, 114: 84-89.
|
| [6] |
Jing H, Liu Z H, Wu B, et al. Physiological and molecular responses to hypoxia stress in Manila clam Ruditapes philippinarum[J]. Aquatic Toxicology, 2023, 257: 106428. doi: 10.1016/j.aquatox.2023.106428
|
| [7] |
周丽青, 井浩, 葛广玉, 等. 溶解氧变化模式对菲律宾蛤仔鳃组织和血淋巴液氧化应激及生理代谢的影响[J]. 中国水产科学, 2023, 30 (3): 361-370. doi: 10.12264/JFSC2022-0401
Zhou L Q, Jing H, Ge G Y, et al. Effects of three dissolved oxygen modes on oxidative stress and physiological metabolism in Ruditapes philippinarum gill tissue and hemolymph[J]. Journal of Fishery Sciences of China, 2023, 30 (3): 361-370 (in Chinese). doi: 10.12264/JFSC2022-0401
|
| [8] |
Feng H M, Chen H Z, Qiang J C, et al. Mechanisms regarding respiratory toxicity triggered by accumulation of ROS in carp exposed to difenoconazole[J]. Pesticide Biochemistry and Physiology, 2023, 191: 105343. doi: 10.1016/j.pestbp.2023.105343
|
| [9] |
王化敏, 丁鉴锋, 杨东敏, 等. 4 种壳色菲律宾蛤仔在低氧胁迫下的耐受能力比较研究[J]. 大连海洋大学学报, 2018, 33 (2): 181-189.
Wang H M, Ding J F, Yang D M, et al. Comparison of hypoxia stress tolerance among Manila clam Ruditapes philippinarum with four shell-colors[J]. Journal of Dalian Ocean University, 2018, 33 (2): 181-189 (in Chinese).
|
| [10] |
王进华, 柏彬彬, 邓婉菲, 等. 镉诱导文蛤鳃细胞凋亡和抗氧化生物标志物的响应[J]. 生态毒理学报, 2022, 17 (2): 413-423.
Wang J H, Bai B B, Deng W F, et al. Apoptosis of Meretrix meretrix gill cell and response of antioxidative biomarkers induced by cadmium[J]. Asian Journal of Ecotoxicology, 2022, 17 (2): 413-423 (in Chinese).
|
| [11] |
孙振兴, 张晾, 郝丽红, 等. 菲律宾蛤仔鳃组织的原代培养[J]. 水产科学, 2004, 23 (2): 12-14. doi: 10.3969/j.issn.1003-1111.2004.02.004
Sun Z X, Zhang L, Hao L H, et al. Primary culture of gill tissue of Ruditapes philippinarum[J]. Fisheries Science, 2004, 23 (2): 12-14 (in Chinese). doi: 10.3969/j.issn.1003-1111.2004.02.004
|
| [12] |
Company R, Serafim A, Cosson R, et al. Temporal variation in the antioxidant defence system and lipid peroxidation in the gills and mantle of hydrothermal vent mussel Bathymodiolus azoricus[J]. Deep-Sea Research Part I, 2006, 53: 1101-1116. doi: 10.1016/j.dsr.2006.05.008
|
| [13] |
Andreyeva A Y, Gostyukhina O L, Kladchenko E S, et al. Acute hypoxic exposure: effect on hemocyte functional parameters and antioxidant potential in gills of the Pacifc oyster, Crassostrea gigas[J]. Marine Environmental Research, 2021, 169: 105389. doi: 10.1016/j.marenvres.2021.105389
|
| [14] |
Li X J, Chen L L, Zhou Z Q, et al. The behavioral and antioxidant response of the bivalve Gomphina veneriformis to sediment burial effect[J]. Acta Oceanologica Sinica, 2021, 40 (6): 75-82. doi: 10.1007/s13131-020-1690-1
|
| [15] |
陈飞飞, 黄桂菊, 油九菊, 等. 合浦珠母贝鳃的显微与超微结构[J]. 动物学杂志, 2012, 47 (6): 78-84.
Chen F F, Huang G J, You J J, et al. Microstructure of the gill in the pearl oyster Pinctada fucata[J]. Chinese Journal of Zoology, 2012, 47 (6): 78-84 (in Chinese).
|
| [16] |
夏玉莹, 张继红, 刘毅. 低氧胁迫下虾夷扇贝的行为特征及生理生化响应[J]. 中国水产科学, 2021, 28 (10): 1319-1328. doi: 10.12264/JFSC2021-0170
Xia Y Y, Zhang J H, Liu Y. Behavioral characteristics and physiological responses to hypoxic stress in Patinopecten yessoensis[J]. Journal of Fishery Sciences of China, 2021, 28 (10): 1319-1328 (in Chinese). doi: 10.12264/JFSC2021-0170
|
| [17] |
郭思鹏, 汪桂玲, 白志毅, 等. 雌性三角帆蚌外鳃组织形态周年变化及低氧胁迫对外鳃组织形态和酶活性影响[J]. 上海海洋大学学报, 2022, 31 (4): 865-872.
Guo S P, Wang G L, Bai Z Y, et al. Annual changes of outer gill tissue morphology and effects of hypoxia stress on outer gill tissue morphology and enzyme activities in female Hyriopsis cumingii[J]. Journal of Shanghai Ocean University, 2022, 31 (4): 865-872 (in Chinese).
|
| [18] |
张倩鸿, 王绍军, 田莹, 等. 溶解氧对香螺行为、抗氧化酶活性及组织结构的影响[J]. 大连海洋大学学报, 2022, 37 (4): 643-649.
Zhang Q H, Wang S J, Tian Y, et al. Effects of dissolved oxygen concentration on behavior, antioxidant enzyme activities and tissue structure of whelk Neptunea cumingii Crosse[J]. Journal of Dalian Ocean University, 2022, 37 (4): 643-649 (in Chinese).
|
| [19] |
Maisano M, Natalotto A, Cappello T, et al. Influence of environmental variables on neurotransmission, oxidative system, and hypoxia signaling on two clam species from a Mediterranean coastal lagoon[J]. Journal of Shellfish Research, 2016, 35 (1): 41-49. doi: 10.2983/035.035.0106
|
| [20] |
Hao Y, Sun C Y, Rong Y, et al. Circulatory and metabolic physiology disorder in different organs of the subtropical scallop species chlamys nobilis under thermal and hypoxia stress, revealed by doppler ultrasonography technique[J]. Frontiers in Marine Science, 2022, 9: 880112. doi: 10.3389/fmars.2022.880112
|
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