Study on the stability of SCC-37 acclimated at 37 °C and its application in common virus culture

The SCC-37 cell line was established by acclimation, the impact of acclimation on cell characteristics was analyzed, and the sensitivity of cells to viruses was assessed. Thereby reducing culture costs and establishing the groundwork for mass production of vaccines. In this experiment, the spinal cord tissue cell line of mandarin fish, Siniperca chuatsi, was used to establish a continuous cell line by a gradual acclimation process with a serum gradient. The origin of the cell line, mycoplasma contamination, growth characteristics, and viability after cryopreservation were analyzed. Additionally, the sensitivity to infectious spleen and kidney necrosis virus (ISKNV), S. chuatsi ranavirus (SCRaV), and Siniperca chuatsi rhabdovirus (SCRV) was evaluated. The cell line, named SCC-37, has been cultured for up to 30 passages in complete medium with L-15+10%NBCS at 37 °C. The S. chuatsi origin of SCC-37 was confirmed by the S. chuatsi 28S rRNA gene. Mycoplasma testing verified that SCC-37 was free of mycoplasma contamination. Sensitivity experiments indicated that ISKNV, SCRaV, and SCRV can steadily proliferate and be passaged in SCC-37. The virus titers were between 10^3.4 and 10^9.6 TCID₅₀/ml in SCC-37. Electron microscopy of SCC-37 showed a large number of virus particles. In summary, the newly acclimated SCC-37 provides a crucial tool for virus isolation, identification and amplification, as well as for vaccine preparation. Its diverse applications warrant further investigation.