Proteomics of liver tissue of large yellow croaker (Larimichthys crocea) under high temperature stress

In order to explore the changes in protein expression of Larimichthys crocea under high temperature stress, tandem mass tag (TMT) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to perform proteomic analysis on the liver tissues between the high temperature tolerant group and the high temperature sensitive group of L. crocea. A total of 3 369 proteins were identified, of which 687 were differentially expressed proteins, including 281 up-regulated proteins and 406 down-regulated proteins. 13 randomly selected proteins were validated with parallel reaction monitoring (PRM) assays, which showed that they were consistent with the TMT-labeled proteomic analysis results. Subsequently, through bioinformatics analysis, it was found that high temperature stress had a significant impact on the protein folding and energy metabolism of L. crocea. Among them, heat shock protein 70(HSP70), calreticulin(CRT), and glucose-regulated protein(GRP) are involved in regulating the correct folding of proteins. Pyruvate kinase(PK) is involved in energy supply under high temperature conditions. It is speculated that HSP70, CRT, GRP and PK play an important role in the response of L. crocea to high temperature stress. This study is helpful to further analyze the high temperature adaptation mechanism of L. crocea, and provides reference for breeding high temperature resistant varieties of L. crocea.